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Capacity for mitochondrial uncoupling and mitochondrial content regress in parallel during cold deacclimation (A) Schematic summary of the time points for sample collection. C57BL/6J mice were acclimated to the cold (4°C) for 7 days with ad libitum access to food and water and subsequently transferred to thermoneutrality (30°C) for 3 h, 12 h, 24 h, or 48 h. Diagram created with BioRender.com . <t>(B)</t> <t>High-resolution</t> respiratory flux per mg of saponin-permeabilized iBAT. Uncoupled respiration was measured in the presence of oligomycin and octanoylcarnitine. Complex I (CI) leak respiration was measured after the addition of malate-pyruvate-glutamate, and CI + CII leak and CI + II + G3P leak respiration were measured following the sequential additions of succinate and glycerol-3-phosphate for respectively, ( n = 16–18/group). (C) Immunoblotting of UCP1 protein expression ( n = 8/group). (D) Quantitative PCR (qPCR) of Ucp1 gene expression, ( n = 9/group). (E) BAT protein concentration (per mg tissue) was measured using a BCA assay in protein lysates, ( n = 10/group). (F) qPCR was used to determine the mtDNA:nDNA ratio (mt-ND1:n-HK2) ( n = 7/group). (G–J) TEM images were analyzed by quantitative morphometry for (G) mitochondrial surface area and (H) lipid droplet surface area, (J) Representative TEM images, ( n = 5 TEM images from 4 mice/group). Scale bars represent 2 μm. See also . Comparisons between time points were determined using a one-way ANOVA with Tukey post-hoc tests, ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001. All values are presented as means ± SD.
High Resolution Respirometry, supplied by Oroboros Instruments, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gnaiger e oroboros o2k fluorespirometer high resolution respirometry o2k calibration  (Oroboros Instruments)
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Oroboros Instruments gnaiger e oroboros o2k fluorespirometer high resolution respirometry o2k calibration
Capacity for mitochondrial uncoupling and mitochondrial content regress in parallel during cold deacclimation (A) Schematic summary of the time points for sample collection. C57BL/6J mice were acclimated to the cold (4°C) for 7 days with ad libitum access to food and water and subsequently transferred to thermoneutrality (30°C) for 3 h, 12 h, 24 h, or 48 h. Diagram created with BioRender.com . <t>(B)</t> <t>High-resolution</t> respiratory flux per mg of saponin-permeabilized iBAT. Uncoupled respiration was measured in the presence of oligomycin and octanoylcarnitine. Complex I (CI) leak respiration was measured after the addition of malate-pyruvate-glutamate, and CI + CII leak and CI + II + G3P leak respiration were measured following the sequential additions of succinate and glycerol-3-phosphate for respectively, ( n = 16–18/group). (C) Immunoblotting of UCP1 protein expression ( n = 8/group). (D) Quantitative PCR (qPCR) of Ucp1 gene expression, ( n = 9/group). (E) BAT protein concentration (per mg tissue) was measured using a BCA assay in protein lysates, ( n = 10/group). (F) qPCR was used to determine the mtDNA:nDNA ratio (mt-ND1:n-HK2) ( n = 7/group). (G–J) TEM images were analyzed by quantitative morphometry for (G) mitochondrial surface area and (H) lipid droplet surface area, (J) Representative TEM images, ( n = 5 TEM images from 4 mice/group). Scale bars represent 2 μm. See also . Comparisons between time points were determined using a one-way ANOVA with Tukey post-hoc tests, ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001. All values are presented as means ± SD.
Gnaiger E Oroboros O2k Fluorespirometer High Resolution Respirometry O2k Calibration, supplied by Oroboros Instruments, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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o2k high resolution respirometry system  (Oroboros Instruments)
86
Oroboros Instruments o2k high resolution respirometry system
Capacity for mitochondrial uncoupling and mitochondrial content regress in parallel during cold deacclimation (A) Schematic summary of the time points for sample collection. C57BL/6J mice were acclimated to the cold (4°C) for 7 days with ad libitum access to food and water and subsequently transferred to thermoneutrality (30°C) for 3 h, 12 h, 24 h, or 48 h. Diagram created with BioRender.com . <t>(B)</t> <t>High-resolution</t> respiratory flux per mg of saponin-permeabilized iBAT. Uncoupled respiration was measured in the presence of oligomycin and octanoylcarnitine. Complex I (CI) leak respiration was measured after the addition of malate-pyruvate-glutamate, and CI + CII leak and CI + II + G3P leak respiration were measured following the sequential additions of succinate and glycerol-3-phosphate for respectively, ( n = 16–18/group). (C) Immunoblotting of UCP1 protein expression ( n = 8/group). (D) Quantitative PCR (qPCR) of Ucp1 gene expression, ( n = 9/group). (E) BAT protein concentration (per mg tissue) was measured using a BCA assay in protein lysates, ( n = 10/group). (F) qPCR was used to determine the mtDNA:nDNA ratio (mt-ND1:n-HK2) ( n = 7/group). (G–J) TEM images were analyzed by quantitative morphometry for (G) mitochondrial surface area and (H) lipid droplet surface area, (J) Representative TEM images, ( n = 5 TEM images from 4 mice/group). Scale bars represent 2 μm. See also . Comparisons between time points were determined using a one-way ANOVA with Tukey post-hoc tests, ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001. All values are presented as means ± SD.
O2k High Resolution Respirometry System, supplied by Oroboros Instruments, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/o2k high resolution respirometry system/product/Oroboros Instruments
Average 86 stars, based on 1 article reviews
o2k high resolution respirometry system - by Bioz Stars, 2026-05
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oroboros o2k high resolution respirometry  (Oroboros Instruments)
86
Oroboros Instruments oroboros o2k high resolution respirometry
Capacity for mitochondrial uncoupling and mitochondrial content regress in parallel during cold deacclimation (A) Schematic summary of the time points for sample collection. C57BL/6J mice were acclimated to the cold (4°C) for 7 days with ad libitum access to food and water and subsequently transferred to thermoneutrality (30°C) for 3 h, 12 h, 24 h, or 48 h. Diagram created with BioRender.com . <t>(B)</t> <t>High-resolution</t> respiratory flux per mg of saponin-permeabilized iBAT. Uncoupled respiration was measured in the presence of oligomycin and octanoylcarnitine. Complex I (CI) leak respiration was measured after the addition of malate-pyruvate-glutamate, and CI + CII leak and CI + II + G3P leak respiration were measured following the sequential additions of succinate and glycerol-3-phosphate for respectively, ( n = 16–18/group). (C) Immunoblotting of UCP1 protein expression ( n = 8/group). (D) Quantitative PCR (qPCR) of Ucp1 gene expression, ( n = 9/group). (E) BAT protein concentration (per mg tissue) was measured using a BCA assay in protein lysates, ( n = 10/group). (F) qPCR was used to determine the mtDNA:nDNA ratio (mt-ND1:n-HK2) ( n = 7/group). (G–J) TEM images were analyzed by quantitative morphometry for (G) mitochondrial surface area and (H) lipid droplet surface area, (J) Representative TEM images, ( n = 5 TEM images from 4 mice/group). Scale bars represent 2 μm. See also . Comparisons between time points were determined using a one-way ANOVA with Tukey post-hoc tests, ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001. All values are presented as means ± SD.
Oroboros O2k High Resolution Respirometry, supplied by Oroboros Instruments, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Capacity for mitochondrial uncoupling and mitochondrial content regress in parallel during cold deacclimation (A) Schematic summary of the time points for sample collection. C57BL/6J mice were acclimated to the cold (4°C) for 7 days with ad libitum access to food and water and subsequently transferred to thermoneutrality (30°C) for 3 h, 12 h, 24 h, or 48 h. Diagram created with BioRender.com . (B) High-resolution respiratory flux per mg of saponin-permeabilized iBAT. Uncoupled respiration was measured in the presence of oligomycin and octanoylcarnitine. Complex I (CI) leak respiration was measured after the addition of malate-pyruvate-glutamate, and CI + CII leak and CI + II + G3P leak respiration were measured following the sequential additions of succinate and glycerol-3-phosphate for respectively, ( n = 16–18/group). (C) Immunoblotting of UCP1 protein expression ( n = 8/group). (D) Quantitative PCR (qPCR) of Ucp1 gene expression, ( n = 9/group). (E) BAT protein concentration (per mg tissue) was measured using a BCA assay in protein lysates, ( n = 10/group). (F) qPCR was used to determine the mtDNA:nDNA ratio (mt-ND1:n-HK2) ( n = 7/group). (G–J) TEM images were analyzed by quantitative morphometry for (G) mitochondrial surface area and (H) lipid droplet surface area, (J) Representative TEM images, ( n = 5 TEM images from 4 mice/group). Scale bars represent 2 μm. See also . Comparisons between time points were determined using a one-way ANOVA with Tukey post-hoc tests, ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001. All values are presented as means ± SD.

Journal: iScience

Article Title: Functional and metabolomic analyses of brown adipose tissue during cold-deacclimation reveal rapid N-acetylated amino acid adaptations

doi: 10.1016/j.isci.2026.115146

Figure Lengend Snippet: Capacity for mitochondrial uncoupling and mitochondrial content regress in parallel during cold deacclimation (A) Schematic summary of the time points for sample collection. C57BL/6J mice were acclimated to the cold (4°C) for 7 days with ad libitum access to food and water and subsequently transferred to thermoneutrality (30°C) for 3 h, 12 h, 24 h, or 48 h. Diagram created with BioRender.com . (B) High-resolution respiratory flux per mg of saponin-permeabilized iBAT. Uncoupled respiration was measured in the presence of oligomycin and octanoylcarnitine. Complex I (CI) leak respiration was measured after the addition of malate-pyruvate-glutamate, and CI + CII leak and CI + II + G3P leak respiration were measured following the sequential additions of succinate and glycerol-3-phosphate for respectively, ( n = 16–18/group). (C) Immunoblotting of UCP1 protein expression ( n = 8/group). (D) Quantitative PCR (qPCR) of Ucp1 gene expression, ( n = 9/group). (E) BAT protein concentration (per mg tissue) was measured using a BCA assay in protein lysates, ( n = 10/group). (F) qPCR was used to determine the mtDNA:nDNA ratio (mt-ND1:n-HK2) ( n = 7/group). (G–J) TEM images were analyzed by quantitative morphometry for (G) mitochondrial surface area and (H) lipid droplet surface area, (J) Representative TEM images, ( n = 5 TEM images from 4 mice/group). Scale bars represent 2 μm. See also . Comparisons between time points were determined using a one-way ANOVA with Tukey post-hoc tests, ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001. All values are presented as means ± SD.

Article Snippet: Oxygen consumption was quantified in BAT using high-resolution respirometry (O2K, Oroboros Instruments, Innsbruck, Austria).

Techniques: Western Blot, Expressing, Real-time Polymerase Chain Reaction, Gene Expression, Protein Concentration, BIA-KA